Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 452.40 | p-Rb(S807/S811) | 159.51 | p-ERK(T202/Y204) | 579.26 | 1 | Colo858-signal1 | B10 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 479.59 | p-Rb(S807/S811) | 159.95 | p-ERK(T202/Y204) | 654.45 | 1 | Colo858-signal1 | B11 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1101.80 | p-Rb(S807/S811) | 48.19 | p-ERK(T202/Y204) | 9.79 | 1 | Colo858-signal1 | B2 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1016.50 | p-Rb(S807/S811) | 42.64 | p-ERK(T202/Y204) | 14.04 | 1 | Colo858-signal1 | B3 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1063.20 | p-Rb(S807/S811) | 48.29 | p-ERK(T202/Y204) | 17.48 | 1 | Colo858-signal1 | B4 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 964.47 | p-Rb(S807/S811) | 50.08 | p-ERK(T202/Y204) | 25.09 | 1 | Colo858-signal1 | B5 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 849.44 | p-Rb(S807/S811) | 79.57 | p-ERK(T202/Y204) | 75.64 | 1 | Colo858-signal1 | B6 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 555.61 | p-Rb(S807/S811) | 125.96 | p-ERK(T202/Y204) | 272.64 | 1 | Colo858-signal1 | B7 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 496.15 | p-Rb(S807/S811) | 149.24 | p-ERK(T202/Y204) | 495.70 | 1 | Colo858-signal1 | B8 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 458.31 | p-Rb(S807/S811) | 151.87 | p-ERK(T202/Y204) | 580.80 | 1 | Colo858-signal1 | B9 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 489.20 | p-Rb(S807/S811) | 160.66 | p-ERK(T202/Y204) | 628.14 | 2 | Colo858-signal1 | C10 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 452.92 | p-Rb(S807/S811) | 154.74 | p-ERK(T202/Y204) | 648.89 | 2 | Colo858-signal1 | C11 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1100.60 | p-Rb(S807/S811) | 49.89 | p-ERK(T202/Y204) | 9.41 | 2 | Colo858-signal1 | C2 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1027.10 | p-Rb(S807/S811) | 46.02 | p-ERK(T202/Y204) | 14.33 | 2 | Colo858-signal1 | C3 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1014.60 | p-Rb(S807/S811) | 48.01 | p-ERK(T202/Y204) | 18.67 | 2 | Colo858-signal1 | C4 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 1028.10 | p-Rb(S807/S811) | 48.93 | p-ERK(T202/Y204) | 27.49 | 2 | Colo858-signal1 | C5 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 904.25 | p-Rb(S807/S811) | 65.93 | p-ERK(T202/Y204) | 58.28 | 2 | Colo858-signal1 | C6 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 597.47 | p-Rb(S807/S811) | 132.94 | p-ERK(T202/Y204) | 291.40 | 2 | Colo858-signal1 | C7 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 483.48 | p-Rb(S807/S811) | 154.86 | p-ERK(T202/Y204) | 515.79 | 2 | Colo858-signal1 | C8 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 461.92 | p-Rb(S807/S811) | 163.29 | p-ERK(T202/Y204) | 613.35 | 2 | Colo858-signal1 | C9 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 438.30 | p-Rb(S807/S811) | 160.10 | p-ERK(T202/Y204) | 626.02 | 1 | Colo858-signal1 | D10 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 454.18 | p-Rb(S807/S811) | 159.65 | p-ERK(T202/Y204) | 628.56 | 1 | Colo858-signal1 | D11 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 738.89 | p-Rb(S807/S811) | 249.29 | p-ERK(T202/Y204) | 766.04 | 1 | Colo858-signal1 | D2 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 596.02 | p-Rb(S807/S811) | 233.59 | p-ERK(T202/Y204) | 707.32 | 1 | Colo858-signal1 | D3 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 488.74 | p-Rb(S807/S811) | 190.14 | p-ERK(T202/Y204) | 694.83 | 1 | Colo858-signal1 | D4 |