| HMS LINCS ID:
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51140
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| Name:
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MGH312
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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| Parent Cell Line:
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| Reference Source:
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Massachusetts General Hospital
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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epithelial
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| Details of Cell Type:
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| Donor Sex:
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female
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| Donor Age:
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| Donor Ethnicity:
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| Donor Health Status:
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| Disease:
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| Details of Disease:
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| Production Details:
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Cell line established from liver metastasis
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| Genetic Modification(s):
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none
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| Known Mutations:
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| Citation Information for Mutations:
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| Verification Reference Profile:
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| Growth Properties:
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adherent
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| Recommended Culture Conditions:
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Cells are cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillin/streptomycin. Protocol: Aspirate spent medium, wash cells once with PBS, trypsinize cells with 0.25% trypsin. Suspend the trypsinized cells in growth media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:4 recommended.
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| Relevant Citations:
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| Usage Note:
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| Comments:
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Established at MGH from patient sample
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| Date Publicly Available:
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2020-03-02
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| Most Recent Update:
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2020-03-02
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