MDAMB330 - Cell Line (ID:51139)
HMS LINCS ID: | 51139 |
Name: | MDAMB330 |
Alternative Names: | |
LINCS ID: | |
Alternative ID: | |
Parent Cell Line: | |
Reference Source: | ATCC HTB-127 |
Organism: | Homo sapiens |
Organ: | breast |
Tissue: | |
Cell Type: | epithelial |
Details of Cell Type: | |
Donor Sex: | female |
Donor Age: | |
Donor Ethnicity: | |
Donor Health Status: | |
Disease: | |
Details of Disease: | |
Production Details: | |
Genetic Modification(s): | none |
Known Mutations: | |
Citation Information for Mutations: | 1330941 |
Verification Reference Profile: | |
Growth Properties: | adherent |
Recommended Culture Conditions: | From the vendor: Cells are cultured in Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with: 30 ng/ ml human recombinant EGF, 0.016 mg/ml human recombinant insulin, 2mM glutathione, 20% fetal bovine serum. Protocol: Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and let the culture sit at 37C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. A subcultivation ratio of 1:2 to 1:4 is recommended. Maintain cultures at 37˚C free of CO2. |
Relevant Citations: | |
Usage Note: | |
Comments: | |
Date Publicly Available: | 2020-03-02 |
Most Recent Update: | 2020-03-02 |
Datasets:
HMS Dataset ID | Dataset Title | HMS Dataset Type |
---|---|---|
20370 | Breast Cancer Profiling Project – Proteomics 3: 1 total proteome dataset for a 27-cell line breast cancer panel under basal conditions | Proteomics |
Batch Information:
HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
---|---|---|---|
51139-1 | ATCC | HTB-127 |