EVSA-T - Cell Line (ID:51138)
HMS LINCS ID: | 51138 |
Name: | EVSA-T |
Alternative Names: | |
LINCS ID: | |
Alternative ID: | |
Parent Cell Line: | |
Reference Source: | Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 433 |
Organism: | Homo sapiens |
Organ: | breast |
Tissue: | |
Cell Type: | epithelial |
Details of Cell Type: | |
Donor Sex: | female |
Donor Age: | |
Donor Ethnicity: | |
Donor Health Status: | |
Disease: | |
Details of Disease: | |
Production Details: | |
Genetic Modification(s): | none |
Known Mutations: | |
Citation Information for Mutations: | 906862 |
Verification Reference Profile: | |
Growth Properties: | adherent |
Recommended Culture Conditions: | From the vendor: Cells are cultured in EMEM + 10% h.i. FBS + 2 mM L-glutamine. Protocol: split semi-confluent culture 1:3 every 3-4 days using trypsin/EDTA, dissociate cells with a pipette (10 ml) during trypsination; seed out at ~ 2 x 10^6 cells/25 cm2 in 10 ml medium; maximal density at ~0.6 x 10^6/cm2; after thawing, viability may be low (about 50%) for the first week. Maintain cultures at 37 °C with 5% CO2. |
Relevant Citations: | |
Usage Note: | |
Comments: | |
Date Publicly Available: | 2020-03-02 |
Most Recent Update: | 2020-03-02 |
Batch Information for HMSL51138-1:
HMS LINCS Batch ID: | 51138-1 |
Provider: | Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures |
Provider Catalog ID: | ACC 433 |
Provider Batch ID: | |
Source Information: | |
Date Received: | |
HMS QC Outcome: | |
Transient Modification(s): | |
Comments: | |
Date Publicly Available: | 2020-03-02 |
Most Recent Update: | 2020-03-02 |