PDX1206 - Cell Line (ID:51137)
| HMS LINCS ID: | 51137 |
| Name: | PDX1206 |
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| Parent Cell Line: | |
| Reference Source: | Caitlin Mills (Harvard Medical School) |
| Organism: | Homo sapiens |
| Organ: | breast |
| Tissue: | |
| Cell Type: | epithelial |
| Details of Cell Type: | |
| Donor Sex: | female |
| Donor Age: | |
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| Production Details: | |
| Genetic Modification(s): | none |
| Known Mutations: | |
| Citation Information for Mutations: | |
| Verification Reference Profile: | DNA Profile (STR): Amelogenin: X CSF1PO: 11 D13S317: 7 D16S539: 13 D5S818: 13 D7S820: 9,10 THO1: 8 TPOX: 8,11 vWA: 16 |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | Cells are cultured in F-media: 3:1 mix of complete Dulbecco's MEM (containing 10% fetal bovine serum, 1% penicillin/streptomycin) and Ham's F12 suplemented with 0.125 ng/ml epidermal growth factor, 25 ng/ml hydrocortisone, 5 ug/ml insulin, 8.6 ng/ml cholera toxin, and 5 uM Y-27632 (HMSLID 10176). Protocol: Collect spent culture medium in a falcon tube, wash cells once with PBS and collect wash, trypsinize cells with 0.25% trypsin, collect trypsinized cells. Centrifuge at 300 x g for 5 min. Resuspend the cell pellet in F-media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:4 recommended. |
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| Comments: | Established by Caitlin Mills (Harvard Medical School) |
| Date Publicly Available: | 2020-03-02 |
| Most Recent Update: | 2020-03-02 |
Batch Information for HMSL51137-1:
| HMS LINCS Batch ID: | 51137-1 |
| Provider: | Caitlin Mills (Harvard Medical School) |
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| Date Received: | |
| HMS QC Outcome: | |
| Transient Modification(s): | |
| Comments: | |
| Date Publicly Available: | 2020-03-02 |
| Most Recent Update: | 2020-03-02 |