| HMS LINCS ID:
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51136
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| Name:
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PDXHCI002
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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| Parent Cell Line:
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| Reference Source:
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Dan Stover (Harvard Medical School)
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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epithelial
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| Details of Cell Type:
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| Donor Sex:
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female
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| Donor Age:
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| Donor Ethnicity:
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| Donor Health Status:
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| Disease:
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DOID: 3008, IDC
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| Details of Disease:
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poorly differentiated, medullary type, stage 3A
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| Production Details:
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| Genetic Modification(s):
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none
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| Known Mutations:
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| Citation Information for Mutations:
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| Verification Reference Profile:
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DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 9,12 D16S539: 12 D5S818: 12 D7S820: 8,11 THO1: 6,8 TPOX: 8,11 vWA: 17,19
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| Growth Properties:
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adherent
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| Recommended Culture Conditions:
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Cells are cultured in F-media: 3:1 mix of complete Dulbecco's MEM (containing 10% fetal bovine serum, 1% penicillin/streptomycin) and Ham's F12 suplemented with 0.125 ng/ml epidermal growth factor, 25 ng/ml hydrocortisone, 5 ug/ml insulin, 8.6 ng/ml cholera toxin, and 5 uM Y-27632 (HMSLID 10176). Protocol: Aspirate spent culture medium, wash cells once with PBS, trypsinize cells with 0.25% trypsin, collect trypsinized cells. Centrifuge at 300 x g for 5 min. Resuspend the cell pellet in F-media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:6 recommended.
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| Relevant Citations:
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PMID 22019887
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| Usage Note:
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| Comments:
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Established by Dan Stover (Joan Brugge Lab, Harvard Medical School) from a PDX tumor (Alana Welm Lab, University of Utah)
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| Date Publicly Available:
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2018-03-27
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| Most Recent Update:
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2018-03-27
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