HMS LINCS ID:
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51134
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Name:
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PDX1258
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Alternative Names:
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LINCS ID:
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Alternative ID:
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Parent Cell Line:
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Reference Source:
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Dan Stover (Harvard Medical School)
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Organism:
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Homo sapiens
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Organ:
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breast
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Tissue:
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Cell Type:
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epithelial
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Details of Cell Type:
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Donor Sex:
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female
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Donor Age:
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Donor Ethnicity:
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Donor Health Status:
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Disease:
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Details of Disease:
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metaplastic triple negative breast cancer
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Production Details:
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Genetic Modification(s):
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none
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Known Mutations:
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Citation Information for Mutations:
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Verification Reference Profile:
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DNA Profile (STR): Amelogenin: X CSF1PO: 11 D13S317: 7 D16S539: 13 D5S818: 13 D7S820: 9,10 THO1: 8 TPOX: 8,11 vWA: 16
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Growth Properties:
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adherent
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Recommended Culture Conditions:
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Cells are cultured in F-media: 3:1 mix of complete Dulbecco's MEM (containing 10% fetal bovine serum, 1% penicillin/streptomycin) and Ham's F12 suplemented with 0.125 ng/ml epidermal growth factor, 25 ng/ml hydrocortisone, 5 ug/ml insulin, 8.6 ng/ml cholera toxin, and 5 uM Y-27632 (HMSLID 10176). Protocol: Collect spent culture medium in a falcon tube, wash cells once with PBS and collect wash, trypsinize cells with 0.25% trypsin, collect trypsinized cells. Centrifuge at 300 x g for 5 min. Resuspend the cell pellet in F-media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:4 recommended.
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Relevant Citations:
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Usage Note:
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Comments:
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Established by Dan Stover (Joan Brugge Lab, Harvard Medical School) from a PDX tumor (Jean Zhao Lab, Dana Farber Cancer Institute, established by Elgene Lim)
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Date Publicly Available:
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2018-03-27
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Most Recent Update:
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2018-03-27
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