HMS LINCS ID:
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51133
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Name:
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MCF 10A EKAREV H2B-mCherry
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Alternative Names:
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LINCS ID:
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Alternative ID:
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CLO_0007599
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Parent Cell Line:
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HMSL50583-13: MCF 10A
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Reference Source:
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Somponnat Sampattavanich (Mahidol University)
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Organism:
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Homo sapiens
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Organ:
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breast
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Tissue:
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Cell Type:
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epithelial
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Details of Cell Type:
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Donor Sex:
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female
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Donor Age:
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Donor Ethnicity:
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Donor Health Status:
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Disease:
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fibrocystic disease
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Details of Disease:
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non-tumorigenic
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Production Details:
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Modification type: stable retroviral transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated constructs: an H2B-mCherry expression cassette and pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing the ERK FRET/CFP reporter EKAREV (PMID: 23219535; PMID: 24140422)
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Genetic Modification(s):
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pPB-CAG-EKAREV
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Known Mutations:
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Citation Information for Mutations:
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Verification Reference Profile:
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Growth Properties:
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adherent
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Recommended Culture Conditions:
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Relevant Citations:
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Usage Note:
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Comments:
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Date Publicly Available:
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2017-05-02
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Most Recent Update:
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2017-05-02
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