| HMS LINCS ID:
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51132
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| Name:
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MCF 10A EKAREV-P2A-F3aN400-mCherry
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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CLO_0007599
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| Parent Cell Line:
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HMSL50583-12: MCF 10A
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| Reference Source:
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Somponnat Sampattavanich (Mahidol University)
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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epithelial
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| Details of Cell Type:
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| Donor Sex:
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female
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| Donor Age:
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| Donor Ethnicity:
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| Donor Health Status:
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| Disease:
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fibrocystic disease
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| Details of Disease:
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non-tumorigenic
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| Production Details:
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Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing EKAREV-P2A-FoxO3aN400-HA-mCherry, which includes the ERK FRET/CFP reporter EKAREV (PMID: 23219535; PMID: 24140422), a P2A site, and a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479)
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| Genetic Modification(s):
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pPB-CAG-EKAREV-P2A-FoxO3aN400-HA-mCherry
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| Known Mutations:
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| Citation Information for Mutations:
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| Verification Reference Profile:
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| Growth Properties:
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adherent
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| Recommended Culture Conditions:
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| Relevant Citations:
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| Usage Note:
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| Comments:
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| Date Publicly Available:
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2017-05-02
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| Most Recent Update:
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2017-05-02
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