184A1 F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry - Cell Line (ID:51131)

HMS LINCS ID: 51131
Name: 184A1 F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry
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Alternative ID: CLO_0001137
Parent Cell Line: HMSL51088-3: 184A1
Reference Source: Somponnat Sampattavanich (Mahidol University)
Organism: Homo sapiens
Organ: breast
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Production Details: Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated AKT phosphorylation sites T32A/S253A/S315A
Genetic Modification(s): pPB-CAG-FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry
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Growth Properties: adherent
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Date Publicly Available: 2017-05-02
Most Recent Update: 2017-05-02

Batch Information for HMSL51131-1:

HMS LINCS Batch ID: 51131-1
Provider: Somponnat Sampattavanich (Mahidol University)
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Date Publicly Available: 2017-05-02
Most Recent Update: 2017-05-02