HMS LINCS ID:
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51131
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Name:
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184A1 F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry
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Alternative Names:
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LINCS ID:
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Alternative ID:
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CLO_0001137
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Parent Cell Line:
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HMSL51088-3: 184A1
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Reference Source:
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Somponnat Sampattavanich (Mahidol University)
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Organism:
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Homo sapiens
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Organ:
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breast
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Tissue:
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Cell Type:
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Details of Cell Type:
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Donor Sex:
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Donor Age:
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Donor Ethnicity:
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Donor Health Status:
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Disease:
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Details of Disease:
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Production Details:
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Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated AKT phosphorylation sites T32A/S253A/S315A
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Genetic Modification(s):
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pPB-CAG-FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry
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Known Mutations:
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Citation Information for Mutations:
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Verification Reference Profile:
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Growth Properties:
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adherent
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Recommended Culture Conditions:
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Relevant Citations:
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Usage Note:
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Comments:
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Date Publicly Available:
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2017-05-02
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Most Recent Update:
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2017-05-02
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