MCF 10A F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry - Cell Line (ID:51128)
| HMS LINCS ID: | 51128 |
| Name: | MCF 10A F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry |
| Alternative Names: | |
| LINCS ID: | |
| Alternative ID: | CLO_0007599 |
| Parent Cell Line: | HMSL50583-11: MCF 10A |
| Reference Source: | Somponnat Sampattavanich (Mahidol University) |
| Organism: | Homo sapiens |
| Organ: | breast |
| Tissue: | |
| Cell Type: | epithelial |
| Details of Cell Type: | |
| Donor Sex: | female |
| Donor Age: | |
| Donor Ethnicity: | |
| Donor Health Status: | |
| Disease: | fibrocystic disease |
| Details of Disease: | non-tumorigenic |
| Production Details: | Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated AKT phosphorylation sites T32A/S253A/S315A |
| Genetic Modification(s): | pPB-CAG-FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry |
| Known Mutations: | |
| Citation Information for Mutations: | |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | |
| Relevant Citations: | |
| Usage Note: | |
| Comments: | |
| Date Publicly Available: | 2017-05-02 |
| Most Recent Update: | 2017-05-02 |
Datasets:
| HMS Dataset ID | Dataset Title | HMS Dataset Type |
|---|---|---|
| 20290 | Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 1 of 3: single-cell reporter measurements. | Microscopy/Imaging |
| 20291 | Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 2 of 3: single-cell reporter pulsing metrics. | Analysis |
| 20292 | Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 3 of 3: mean reporter pulsing metrics. | Analysis |
Batch Information:
| HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
|---|---|---|---|
| 51128-1 | Somponnat Sampattavanich (Mahidol University) |