MCF 10A F3aN400S294A/S344A-Venus-P2A-NLS-mCherry - Cell Line (ID:51127)

Name: MCF 10A F3aN400S294A/S344A-Venus-P2A-NLS-mCherry
Alternative Names:
Alternative ID: CLO_0007599
Parent Cell Line: HMSL50583-11: MCF 10A
Reference Source: Somponnat Sampattavanich (Mahidol University)
Organism: Homo sapiens
Organ: breast
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: fibrocystic disease
Details of Disease: non-tumorigenic
Production Details: Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400S294A/S344A-HA-Venus-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated ERK phosphorylation sites S294A/S344A
Genetic Modification(s): pPB-CAG-FoxO3aN400S294A/S344A-HA-Venus-P2A-NLS-Myc-mCherry
Known Mutations:
Citation Information for Mutations:
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions:
Relevant Citations:
Usage Note:
Date Publicly Available: 2017-05-02
Most Recent Update: 2017-05-02

Batch Information for HMSL51127-1:

HMS LINCS Batch ID: 51127-1
Provider: Somponnat Sampattavanich (Mahidol University)
Provider Catalog ID:
Provider Batch ID:
Source Information:
Date Received:
HMS QC Outcome:
Transient Modification(s):
Date Publicly Available: 2017-05-02
Most Recent Update: 2017-05-02