| HMS LINCS ID:
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51125
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| Name:
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184A1 F3aN400-Venus NLS-mCherry
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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CLO_0001137
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| Parent Cell Line:
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HMSL51088-2: 184A1
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| Reference Source:
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Somponnat Sampattavanich (Mahidol University)
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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| Details of Cell Type:
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| Donor Sex:
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| Donor Age:
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| Donor Ethnicity:
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| Donor Health Status:
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| Disease:
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| Details of Disease:
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| Production Details:
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Modification type: stable retroviral transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated constructs: an NLS-mCherry expression cassette and pMSCV-puro-FoxO3aN400-Venus, a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) inserted upstream of the Venus sequence in pMSCV-puro.
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| Genetic Modification(s):
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NLS-mCherry and pMSCV-puro-FoxO3aN400-Venus integration
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| Known Mutations:
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| Citation Information for Mutations:
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| Verification Reference Profile:
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| Growth Properties:
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adherent
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| Recommended Culture Conditions:
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| Relevant Citations:
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| Usage Note:
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| Comments:
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| Date Publicly Available:
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2017-05-02
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| Most Recent Update:
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2017-05-02
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