| HMS LINCS ID:
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51123
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| Name:
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HMT-3522 T4-2
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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| Parent Cell Line:
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| Reference Source:
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European Collection of Authenticated Cell Cultures (ECACC) 98102212
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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epithelial
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| Details of Cell Type:
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| Donor Sex:
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female
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| Donor Age:
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48
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| Donor Ethnicity:
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Caucasian
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| Donor Health Status:
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| Disease:
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DOID:0060082, breast benign neoplasm
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| Details of Disease:
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| Production Details:
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From ECACC: HMT-3522 T4-2, also known as HMT-3522/mt-1, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. A mutation at codon 179 of the p53 gene was detected during establishment of the parent line at passage 25-30. The subline S1 is a continuation of this line from passage 34 (ECACC cataogue no. 98102210) growing without collagen. Subline S2 (ECACC catalogue no. 98102211) has been isolated by culturing S1 cells from passage 118 in serum-free media without epidermal growth factor (EGF). The third subline, T4-2 was established from S2 at passage 238 and is the only tumorigenic line in nude mice out of the 3 sublines.
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| Genetic Modification(s):
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none
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| Known Mutations:
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mutation at codon 179 of P53; trisomy for the short arm of chromosome 7
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| Citation Information for Mutations:
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European Collection of Authenticated Cell Cultures (ECACC) 98102212
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| Verification Reference Profile:
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DNA Profile (STR, source: Asterand): STR-PCR Data: Amelogenin: X CSF1PO: 11,12 D13S317: 11 D16S539: 11,13 D5S818: 9,11 D7S 820: 8,9 THO1: 9,9.3 TPOX: 8,11 vWA: 14,17
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| Growth Properties:
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adherent
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| Recommended Culture Conditions:
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From ECACC: DMEM / Ham's F12 (1:1) + 2 mM Glutamine + 250 ng/ml insulin + 10 µg / ml transferrin +10-8 M Sodium selenite + 10-10 M 17 beta-estradiol + 0.5 µg/ml hydrocortisone + 5 µg / ml ovine prolactin
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| Relevant Citations:
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PMID 3558253
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| Usage Note:
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| Comments:
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| Date Publicly Available:
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2016-08-24
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| Most Recent Update:
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2016-08-24
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