| HMS LINCS ID:
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51119
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| Name:
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HMT-3522 S1
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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| Parent Cell Line:
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| Reference Source:
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European Collection of Authenticated Cell Cultures (ECACC) 98102210
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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epithelial
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| Details of Cell Type:
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| Donor Sex:
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female
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| Donor Age:
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48
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| Donor Ethnicity:
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Caucasian
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| Donor Health Status:
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| Disease:
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DOID:0060082, breast benign neoplasm
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| Details of Disease:
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| Production Details:
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From ECACC: HMT-3522 S1, also known as HMT-3522/wt, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. HMT-3522 S1 is a continuation of this line from passage 34 growing without collagen.
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| Genetic Modification(s):
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none
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| Known Mutations:
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mutation at codon 179 of P53
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| Citation Information for Mutations:
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European Collection of Authenticated Cell Cultures (ECACC) 98102210
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| Verification Reference Profile:
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| Growth Properties:
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adherent
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| Recommended Culture Conditions:
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From ECACC: DMEM / Ham's F12 (1:1) + 2 mM Glutamine + 250 ng/ml insulin + 10 µg/ml transferrin +10-8 M sodium selenite + 10-10 M 17 beta-estradiol + 0.5 µg/ml hydrocortisone + 5 µg/ml ovine prolactin + 10 ng/ml EGF
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| Relevant Citations:
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PMID 3558253
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| Usage Note:
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| Comments:
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| Date Publicly Available:
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2016-08-24
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| Most Recent Update:
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2016-08-24
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