| HMS LINCS ID:
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51114
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| Name:
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EFM-192A
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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CLO_0002889
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| Parent Cell Line:
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| Reference Source:
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Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 258
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
|
|
| Cell Type:
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epithelioid
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| Details of Cell Type:
|
|
| Donor Sex:
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female
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| Donor Age:
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46
|
| Donor Ethnicity:
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Caucasian
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| Donor Health Status:
|
|
| Disease:
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DOID:3458, breast adenocarcinoma
|
| Details of Disease:
|
breast adenocarcinoma
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| Production Details:
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From DSMZ: established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985
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| Genetic Modification(s):
|
none
|
| Known Mutations:
|
ERBB2 amplification
|
| Citation Information for Mutations:
|
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 258
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
adherent; From DSMZ: epitheloid, adherent growing as monolayer
|
| Recommended Culture Conditions:
|
From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine. Subculture: Split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 2 x 106 cells/25 cm2. Incubation: at 37°C with 5% CO2. Doubling time: about 9 days.
|
| Relevant Citations:
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| Usage Note:
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| Comments:
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| Date Publicly Available:
|
2016-08-24
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| Most Recent Update:
|
2017-05-02
|