| HMS LINCS ID:
|
51113
|
| Name:
|
EFM-19
|
| Alternative Names:
|
|
| LINCS ID:
|
|
| Alternative ID:
|
CLO_0002888
|
| Parent Cell Line:
|
|
| Reference Source:
|
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 231
|
| Organism:
|
Homo sapiens
|
| Organ:
|
breast
|
| Tissue:
|
|
| Cell Type:
|
epithelioid
|
| Details of Cell Type:
|
|
| Donor Sex:
|
female
|
| Donor Age:
|
50
|
| Donor Ethnicity:
|
Caucasian
|
| Donor Health Status:
|
|
| Disease:
|
DOID:3007, breast ductal carcinoma
|
| Details of Disease:
|
breast ductal adenocarcinoma
|
| Production Details:
|
From DSMZ: established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma (ductal type, histological grading II) in 1979
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
mixed; From DSMZ: epitheloid, growing in clusters at first, then as monolayer
|
| Recommended Culture Conditions:
|
From DSMZ: Medium: 90% RPMI 1640 + 10% h.i. FBS. Subculture: Split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA. Seed out at ca. 2 x 106 cells/25 cm2. Incubation: at 37°C with 5% CO2. Doubling time: about 64 hours.
|
| Relevant Citations:
|
PMID 6325325
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2016-08-24
|
| Most Recent Update:
|
2017-05-02
|