| HMS LINCS ID:
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51112
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| Name:
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CAL-85-1
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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CLO_0002190
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| Parent Cell Line:
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| Reference Source:
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Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 440
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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epithelial
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| Details of Cell Type:
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| Donor Sex:
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female
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| Donor Age:
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35
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| Donor Ethnicity:
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| Donor Health Status:
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| Disease:
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DOID:3458, breast adenocarcinoma
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| Details of Disease:
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breast adenocarcinoma
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| Production Details:
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From DSMZ: established from the relapsing invasive galactophoric breast adenocarcinoma resected from a 35-year-old woman during radiotherapy in 1990; cells were described as expressing the multidrug resistance (MDR) gene
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| Genetic Modification(s):
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none
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| Known Mutations:
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| Citation Information for Mutations:
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| Verification Reference Profile:
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| Growth Properties:
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adherent; From DSMZ: adherent epithelial-like cells growing in monolayers
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| Recommended Culture Conditions:
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From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate. Subculture: Split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA (incubation with trypsin/EDTA for about 10 min). Seed out at ca. 0.03-0.04 x 106 cells/cm2. Maintain at 0.04 x 106 cells/cm2. A significant amount of cell debris is always visible in the culture background which might be due to granula or other intracellular material. Incubation: at 37°C with 5% CO2. Doubling time: ca. 70 hours.
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| Relevant Citations:
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Fischel, J. L., Formento, P., Lucas, C., Berlion, M., Bizzari, J. P., Milano, G. (1993) Le S9788 dans la réversion de la résistance MDR: Activité comparée à des composés de référence. Bull Cancer 80 : 409.
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| Usage Note:
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| Comments:
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| Date Publicly Available:
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2016-08-24
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| Most Recent Update:
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2016-08-24
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