CAL-85-1 - Cell Line (ID:51112)

HMS LINCS ID: 51112
Name: CAL-85-1
Alternative Names:
LINCS ID:
Alternative ID: CLO_0002190
Parent Cell Line:
Reference Source: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 440
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age: 35
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3458, breast adenocarcinoma
Details of Disease: breast adenocarcinoma
Production Details: From DSMZ: established from the relapsing invasive galactophoric breast adenocarcinoma resected from a 35-year-old woman during radiotherapy in 1990; cells were described as expressing the multidrug resistance (MDR) gene
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations:
Verification Reference Profile:
Growth Properties: adherent; From DSMZ: adherent epithelial-like cells growing in monolayers
Recommended Culture Conditions: From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate. Subculture: Split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA (incubation with trypsin/EDTA for about 10 min). Seed out at ca. 0.03-0.04 x 106 cells/cm2. Maintain at 0.04 x 106 cells/cm2. A significant amount of cell debris is always visible in the culture background which might be due to granula or other intracellular material. Incubation: at 37°C with 5% CO2. Doubling time: ca. 70 hours.
Relevant Citations: Fischel, J. L., Formento, P., Lucas, C., Berlion, M., Bizzari, J. P., Milano, G. (1993) Le S9788 dans la réversion de la résistance MDR: Activité comparée à des composés de référence. Bull Cancer 80 : 409.
Usage Note:
Comments:
Date Publicly Available: 2016-08-24
Most Recent Update: 2016-08-24

Batch Information for HMSL51112-1:

HMS LINCS Batch ID: 51112-1
Provider: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures
Provider Catalog ID: ACC 440
Provider Batch ID:
Source Information: Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ
Date Received:
HMS QC Outcome:
Transient Modification(s):
Comments:
Date Publicly Available: 2016-08-24
Most Recent Update: 2016-08-24