CAL-148 - Cell Line (ID:51111)

HMS LINCS ID: 51111
Name: CAL-148
Alternative Names:
LINCS ID:
Alternative ID: CLO_0002181
Parent Cell Line:
Reference Source: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 460
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type: epithelioid
Details of Cell Type:
Donor Sex: female
Donor Age: 58
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3458, breast adenocarcinoma
Details of Disease: breast adenocarcinoma
Production Details: From DSMZ: established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol)
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations:
Verification Reference Profile:
Growth Properties: mixed; From DSMZ: epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers
Recommended Culture Conditions: From DSMZ: Medium: 80% Dulbecco's MEM + 20% h.i. FBS + 2 mM L-glutamine + EGF (1 µg/100 ml). Subculture: Split confluent culture 1:4 to 1:10 every 3-5 days by agitation. Seed out at ca. 0.2-0.3 x 106 cells/ml (suspension culture) or 0.4-0.8 x 105 cells/cm2 (adherent culture). Maintain at about 0.2-0.7 x 106 cells/ml or equivalent. Cell numbers may be difficult to ascertain due to growth in clumps. Cells grow faster in suspension without EGF. Incubation: at 37°C with 10% CO2. Doubling time: ca. 30-40 hours.
Relevant Citations: PMID 7531416
Usage Note:
Comments:
Date Publicly Available: 2016-08-24
Most Recent Update: 2017-05-02

Batch Information for HMSL51111-1:

HMS LINCS Batch ID: 51111-1
Provider: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures
Provider Catalog ID: ACC 460
Provider Batch ID:
Source Information: Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ
Date Received:
HMS QC Outcome:
Transient Modification(s):
Comments:
Date Publicly Available: 2016-08-24
Most Recent Update: 2016-08-24