| HMS LINCS ID:
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51111
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| Name:
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CAL-148
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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CLO_0002181
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| Parent Cell Line:
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| Reference Source:
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Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 460
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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epithelioid
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| Details of Cell Type:
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| Donor Sex:
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female
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| Donor Age:
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58
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| Donor Ethnicity:
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| Donor Health Status:
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| Disease:
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DOID:3458, breast adenocarcinoma
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| Details of Disease:
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breast adenocarcinoma
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| Production Details:
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From DSMZ: established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol)
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| Genetic Modification(s):
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none
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| Known Mutations:
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| Citation Information for Mutations:
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| Verification Reference Profile:
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| Growth Properties:
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mixed; From DSMZ: epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers
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| Recommended Culture Conditions:
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From DSMZ: Medium: 80% Dulbecco's MEM + 20% h.i. FBS + 2 mM L-glutamine + EGF (1 µg/100 ml). Subculture: Split confluent culture 1:4 to 1:10 every 3-5 days by agitation. Seed out at ca. 0.2-0.3 x 106 cells/ml (suspension culture) or 0.4-0.8 x 105 cells/cm2 (adherent culture). Maintain at about 0.2-0.7 x 106 cells/ml or equivalent. Cell numbers may be difficult to ascertain due to growth in clumps. Cells grow faster in suspension without EGF. Incubation: at 37°C with 10% CO2. Doubling time: ca. 30-40 hours.
|
| Relevant Citations:
|
PMID 7531416
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| Usage Note:
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| Comments:
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| Date Publicly Available:
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2016-08-24
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| Most Recent Update:
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2017-05-02
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