MB 157 - Cell Line (ID:50324)

HMS LINCS ID: 50324
Name: MB 157
Alternative Names:
LINCS ID: LCL-1484
Alternative ID: CLO_0007544
Parent Cell Line:
Reference Source: ATCC CRL-7721
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3459, breast carcinoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations:
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: 1:2 to 1:3
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-07-12

Batch Information for HMSL50324-1:

HMS LINCS Batch ID: 50324-1
Provider: ATCC
Provider Catalog ID: CRL-7721
Provider Batch ID:
Source Information: Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC
Date Received:
HMS QC Outcome:
Transient Modification(s):
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2015-06-29