MB 157 - Cell Line (ID:50324)
| HMS LINCS ID: | 50324 |
| Name: | MB 157 |
| Alternative Names: | |
| LINCS ID: | LCL-1484 |
| Alternative ID: | CLO_0007544 |
| Parent Cell Line: | |
| Reference Source: | ATCC CRL-7721 |
| Organism: | Homo sapiens |
| Organ: | breast |
| Tissue: | |
| Cell Type: | |
| Details of Cell Type: | |
| Donor Sex: | female |
| Donor Age: | |
| Donor Ethnicity: | |
| Donor Health Status: | |
| Disease: | DOID:3459, breast carcinoma |
| Details of Disease: | |
| Production Details: | |
| Genetic Modification(s): | none |
| Known Mutations: | |
| Citation Information for Mutations: | |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: 1:2 to 1:3 |
| Relevant Citations: | |
| Usage Note: | |
| Comments: | |
| Date Publicly Available: | 2012-07-31 |
| Most Recent Update: | 2016-07-12 |
Batch Information for HMSL50324-1:
| HMS LINCS Batch ID: | 50324-1 |
| Provider: | ATCC |
| Provider Catalog ID: | CRL-7721 |
| Provider Batch ID: | |
| Source Information: | Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC |
| Date Received: | |
| HMS QC Outcome: | |
| Transient Modification(s): | |
| Comments: | |
| Date Publicly Available: | 2012-07-31 |
| Most Recent Update: | 2015-06-29 |