HCC1569 - Cell Line (ID:50210)

Name: HCC1569
Alternative Names: HCC-1569
Alternative ID: CLO_0003640
Parent Cell Line:
Reference Source: ATCC CRL-2330
Organism: Homo sapiens
Organ: breast
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3459, breast carcinoma
Details of Disease: breast carcinoma; metastatic carcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS907046
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Batch Information for HMSL50210-1:

HMS LINCS Batch ID: 50210-1
Provider: ATCC
Provider Catalog ID: CRL-2330
Provider Batch ID:
Source Information: Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC
Date Received:
HMS QC Outcome:
Transient Modification(s):
Date Publicly Available: 2012-07-31
Most Recent Update: 2015-06-29