HCC1143 - Cell Line (ID:50205)

Name: HCC1143
Alternative Names:
Alternative ID: CLO_0003630
Parent Cell Line:
Reference Source: ATCC CRL-2321
Organism: Homo sapiens
Organ: breast
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3008, primary ductal carcinoma
Details of Disease: breast ductal carcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS749710
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol: Remove medium, rinse with 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Batch Information for HMSL50205-2:

HMS LINCS Batch ID: 50205-2
Provider: ATCC
Provider Catalog ID: CRL-2321
Provider Batch ID:
Source Information: Received from Kristin Brown (Alex Toker Lab, Beth Israel Deaconess Medical Center)
Date Received:
HMS QC Outcome:
Transient Modification(s):
Date Publicly Available: 2018-02-09
Most Recent Update: 2018-02-09