HCC1143 - Cell Line (ID:50205)
| HMS LINCS ID: | 50205 |
| Name: | HCC1143 |
| Alternative Names: | |
| LINCS ID: | LCL-1329 |
| Alternative ID: | CLO_0003630 |
| Parent Cell Line: | |
| Reference Source: | ATCC CRL-2321 |
| Organism: | Homo sapiens |
| Organ: | breast |
| Tissue: | |
| Cell Type: | |
| Details of Cell Type: | |
| Donor Sex: | female |
| Donor Age: | |
| Donor Ethnicity: | |
| Donor Health Status: | |
| Disease: | DOID:3008, primary ductal carcinoma |
| Details of Disease: | breast ductal carcinoma |
| Production Details: | |
| Genetic Modification(s): | none |
| Known Mutations: | |
| Citation Information for Mutations: | COSS749710 |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol: Remove medium, rinse with 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended |
| Relevant Citations: | |
| Usage Note: | |
| Comments: | |
| Date Publicly Available: | 2012-07-31 |
| Most Recent Update: | 2016-04-04 |
Batch Information for HMSL50205-1:
| HMS LINCS Batch ID: | 50205-1 |
| Provider: | ATCC |
| Provider Catalog ID: | CRL-2321 |
| Provider Batch ID: | |
| Source Information: | Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC |
| Date Received: | |
| HMS QC Outcome: | |
| Transient Modification(s): | |
| Comments: | |
| Date Publicly Available: | 2012-07-31 |
| Most Recent Update: | 2015-06-29 |