| HMS LINCS ID:
|
50149
|
| Name:
|
COLO 858
|
| Alternative Names:
|
|
| LINCS ID:
|
LCL-1242
|
| Alternative ID:
|
CLO_0002569
|
| Parent Cell Line:
|
|
| Reference Source:
|
European Collection of Authenticated Cell Cultures (ECACC) 93052613
|
| Organism:
|
Homo sapiens
|
| Organ:
|
skin
|
| Tissue:
|
|
| Cell Type:
|
|
| Details of Cell Type:
|
|
| Donor Sex:
|
male
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:1909, malignant melanoma
|
| Details of Disease:
|
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37C; 5% CO2.
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-07-31
|
| Most Recent Update:
|
2016-08-24
|