BT-483 - Cell Line (ID:50107)
| HMS LINCS ID: | 50107 |
| Name: | BT-483 |
| Alternative Names: | |
| LINCS ID: | LCL-1309 |
| Alternative ID: | CLO_0002043 |
| Parent Cell Line: | |
| Reference Source: | ATCC HTB-121 |
| Organism: | Homo sapiens |
| Organ: | breast |
| Tissue: | |
| Cell Type: | |
| Details of Cell Type: | |
| Donor Sex: | female |
| Donor Age: | |
| Donor Ethnicity: | |
| Donor Health Status: | |
| Disease: | DOID:3008, ductal carcinoma |
| Details of Disease: | breast ductal carcinoma |
| Production Details: | |
| Genetic Modification(s): | none |
| Known Mutations: | |
| Citation Information for Mutations: | COSS949093 |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 80%; fetal bovine serum, 20%. Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subcultivation ratio: A subcultivation ratio of 1:2 is recommended |
| Relevant Citations: | |
| Usage Note: | |
| Comments: | |
| Date Publicly Available: | 2012-07-31 |
| Most Recent Update: | 2016-04-04 |
Batch Information for HMSL50107-2:
| HMS LINCS Batch ID: | 50107-2 |
| Provider: | ATCC |
| Provider Catalog ID: | HTB-121 |
| Provider Batch ID: | |
| Source Information: | Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010 |
| Date Received: | 2010-07-16 |
| HMS QC Outcome: | |
| Transient Modification(s): | |
| Comments: | |
| Date Publicly Available: | 2016-06-06 |
| Most Recent Update: | 2016-07-12 |