BT-474 - Cell Line (ID:50106)
HMS LINCS ID: | 50106 |
Name: | BT-474 |
Alternative Names: | |
LINCS ID: | LCL-1308 |
Alternative ID: | CLO_0002042 |
Parent Cell Line: | |
Reference Source: | ATCC HTB-20 |
Organism: | Homo sapiens |
Organ: | breast |
Tissue: | |
Cell Type: | |
Details of Cell Type: | |
Donor Sex: | female |
Donor Age: | |
Donor Ethnicity: | |
Donor Health Status: | |
Disease: | DOID:3008, ductal carcinoma |
Details of Disease: | breast ductal carcinoma |
Production Details: | |
Genetic Modification(s): | none |
Known Mutations: | |
Citation Information for Mutations: | COSS946359 |
Verification Reference Profile: | |
Growth Properties: | adherent |
Recommended Culture Conditions: | From MGH/CMT as specified by cell provider: Hybri-Care Medium ATCC 46-X, 90%; fetal bovine serum, 10%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended |
Relevant Citations: | |
Usage Note: | |
Comments: | |
Date Publicly Available: | 2012-07-31 |
Most Recent Update: | 2016-04-04 |
Batch Information for HMSL50106-2:
HMS LINCS Batch ID: | 50106-2 |
Provider: | ATCC |
Provider Catalog ID: | HTB-20 |
Provider Batch ID: | |
Source Information: | Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010 |
Date Received: | 2010-07-16 |
HMS QC Outcome: | |
Transient Modification(s): | |
Comments: | |
Date Publicly Available: | 2016-06-06 |
Most Recent Update: | 2016-07-12 |