AU565 - Cell Line (ID:50091)

HMS LINCS ID: 50091
Name: AU565
Alternative Names: AU-565
LINCS ID: LCL-1462
Alternative ID: CLO_0001769
Parent Cell Line:
Reference Source: ATCC CRL-2351
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3458, adenocarcinoma
Details of Disease: breast adenocarcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS910704
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Subculturing: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Batch Information for HMSL50091-3:

HMS LINCS Batch ID: 50091-3
Provider: ATCC
Provider Catalog ID: CRL-2351
Provider Batch ID:
Source Information: Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010
Date Received: 2010-07-16
HMS QC Outcome:
Transient Modification(s):
Comments:
Date Publicly Available: 2016-06-06
Most Recent Update: 2016-07-12