HeLa - Cell Line (ID:50061)

HMS LINCS ID: 50061
Name: HeLa
Alternative Names:
LINCS ID: LCL-1512
Alternative ID: CLO_0003684
Parent Cell Line:
Reference Source: ATCC CRM-CCL-2
Organism: Homo sapiens
Organ: cervix
Tissue:
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3702, cervical adenocarcinoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS1298134
Verification Reference Profile: DNA Profile (STR, source: ATCC): Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18
Growth Properties: adherent
Recommended Culture Conditions: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 deg C Subculturing protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 deg C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-05-01
Most Recent Update: 2016-07-12

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20094
Torin1 KiNativ -- single dose experiment
KiNativ
20095
Torin2 KiNativ -- single dose experiment
KiNativ
20097
XMD11-50 KiNativ -- single dose experiment
KiNativ
20098
XMD11-85h KiNativ -- single dose experiment
KiNativ
20099
XMD16-144 KiNativ -- single dose experiment
KiNativ
20100
JWE-035 KiNativ -- single dose experiment
KiNativ
20101
XMD8-85 KiNativ -- single dose experiment
KiNativ
20102
XMD8-92 KiNativ -- single dose experiment
KiNativ
20103
XMD-12 KiNativ -- single dose experiment
KiNativ
20105
CH5424802 KiNativ -- single dose experiment
KiNativ
20106
NPK76-II-72-1 KiNativ -- single dose experiment
KiNativ

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
50061-1 ActivX Biosciences, Inc.
50061-2 Suzanne Gaudet (Harvard Medical School)