A-375 - Cell Line (ID:50060)

Name: A-375
Alternative Names: A375
Alternative ID: CLO_0001582
Parent Cell Line:
Reference Source: ATCC CRL-1619
Organism: Homo sapiens
Organ: skin
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:1909, malignant melanoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS906793
Verification Reference Profile: DNA Profile (STR, source: ATCC): Amelogenin: X CSF1PO: 11,12 D13S317: 11,14 D16S539: 9 D5S818: 12 D7S820: 9 THO1: 8 TPOX: 8,10 vWA: 16,17
Growth Properties: adherent
Recommended Culture Conditions: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0 deg C Subculturing protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 deg C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Every 2 to 3 days
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-05-01
Most Recent Update: 2016-07-12

Batch Information for HMSL50060-1:

HMS LINCS Batch ID: 50060-1
Provider: ActivX Biosciences, Inc.
Provider Catalog ID:
Provider Batch ID:
Source Information:
Date Received:
HMS QC Outcome:
Transient Modification(s):
Date Publicly Available: 2012-05-01
Most Recent Update: 2016-07-12