MDA-MB-231 - Cell Line (ID:50058)

Name: MDA-MB-231
Alternative Names: MDAMB231; MDA-MB231
Alternative ID: CLO_0007634
Parent Cell Line:
Reference Source: ATCC HTB-26
Organism: Homo sapiens
Organ: breast
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3458, adenocarcinoma
Details of Disease: breast adenocarcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS905960
Verification Reference Profile: DNA Profile (STR, source: ATCC): Amelogenin: X CSF1PO: 12,13 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 8,9 THO1: 7,9.3 TPOX: 8,9 vWA: 15,18
Growth Properties: adherent
Recommended Culture Conditions: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% Temperature: 37.0 deg C Subculturing protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 deg C without CO2. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-05-01
Most Recent Update: 2016-07-12

Batch Information for HMSL50058-4:

HMS LINCS Batch ID: 50058-4
Provider: ATCC
Provider Catalog ID: HTB-26
Provider Batch ID:
Source Information: Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010
Date Received: 2010-07-16
HMS QC Outcome: PASS
Transient Modification(s):
Date Publicly Available: 2016-06-06
Most Recent Update: 2016-09-09

HMS QC Testing Events for HMSL50058-4:

Date HMS QC Outcome Comment Files
06/30/2015 PASS Chung_STR_Profiling_Results_6_30_15.xls