HME1 - Cell Line (ID:50056)

Name: HME1
Alternative Names: hTERT-HME1
Alternative ID: CLO_0004291
Parent Cell Line:
Reference Source: ATCC CRL-4010
Organism: Homo sapiens
Organ: breast
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: not diseased
Details of Disease:
Production Details: Human mammary epithelium, HME1 cell line, was derived from a 53-year-old patient undergoing reduction mammoplasty surgery (no history of breast cancer). The HME1 cells were immortalized by infection with the retrovirus pBabepuro+hTERT vector and cultured in complete growth medium containing puromycin until stable clones were selected.
Genetic Modification(s): pBabepuro+hTERT integration
Known Mutations:
Citation Information for Mutations:
Verification Reference Profile: DNA Profile (STR, source: ATCC): Amelogenin: X CSF1PO: 10 D13S317: 11,12 D16S539: 11,12 D5S818: 11 D7S820: 7,12 THO1: 7,8 TPOX: 10,12 vWA: 15,16
Growth Properties: adherent
Recommended Culture Conditions: ATCC complete growth medium: The base medium for this cell line (MEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit. Note: Do not filter complete medium Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 deg C Subculturing protocol: Remove and discard culture medium. Add 2.0 to 3.0 ml of 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to the flask and observe cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. To remove trypsin-EDTA solution, add 2.0 to 3.0 ml of 0.1% SoybeanTrypsin Inhibitor solution and aspirate cells by gently pipetting. Transfer cell suspension to a 15 mL centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 7 X 10(3) to 9 X 10(3) viable cells/sq. cm is recommended. Subculture cultures when they reach a cell concentration between 4 X 10(4) and 6 X 10(4) cells/sq. cm. Incubate cultures at 37C. Interval: weekly Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended. Medium Renewal: Every 2 to 3 days
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-05-01
Most Recent Update: 2016-07-12

Batch Information for HMSL50056-1:

HMS LINCS Batch ID: 50056-1
Provider: ATCC
Provider Catalog ID: CRL-4010
Provider Batch ID:
Source Information: Obtained by Mario Niepel (Harvard Medical School) from ATCC in March, 2012
Date Received: 2012-03-02
HMS QC Outcome: PASS
Transient Modification(s):
Date Publicly Available: 2012-05-01
Most Recent Update: 2016-09-09

HMS QC Testing Events for HMSL50056-1:

Date HMS QC Outcome Comment Files
06/30/2015 PASS Chung_STR_Profiling_Results_6_30_15.xls