| HMS LINCS ID:
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50056
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| Name:
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HME1
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| Alternative Names:
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hTERT-HME1
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| LINCS ID:
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LCL-2083
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| Alternative ID:
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CLO_0004291
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| Parent Cell Line:
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| Reference Source:
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ATCC CRL-4010
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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epithelial
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| Details of Cell Type:
|
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| Donor Sex:
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female
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| Donor Age:
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|
| Donor Ethnicity:
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| Donor Health Status:
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| Disease:
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not diseased
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| Details of Disease:
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| Production Details:
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Human mammary epithelium, HME1 cell line, was derived from a 53-year-old patient undergoing reduction mammoplasty surgery (no history of breast cancer).
The HME1 cells were immortalized by infection with the retrovirus pBabepuro+hTERT vector and cultured in complete growth medium containing puromycin until stable clones were selected.
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| Genetic Modification(s):
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pBabepuro+hTERT integration
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| Known Mutations:
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| Citation Information for Mutations:
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| Verification Reference Profile:
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DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 11,12
D5S818: 11
D7S820: 7,12
THO1: 7,8
TPOX: 10,12
vWA: 15,16
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| Growth Properties:
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adherent
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| Recommended Culture Conditions:
|
ATCC complete growth medium: The base medium for this cell line (MEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit. Note: Do not filter complete medium
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Add 2.0 to 3.0 ml of 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to the flask and observe cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
To remove trypsin-EDTA solution, add 2.0 to 3.0 ml of 0.1% SoybeanTrypsin Inhibitor solution and aspirate cells by gently pipetting.
Transfer cell suspension to a 15 mL centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 7 X 10(3) to 9 X 10(3) viable cells/sq. cm is recommended. Subculture cultures when they reach a cell concentration between 4 X 10(4) and 6 X 10(4) cells/sq. cm.
Incubate cultures at 37C.
Interval: weekly
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended.
Medium Renewal: Every 2 to 3 days
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| Relevant Citations:
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| Usage Note:
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| Comments:
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| Date Publicly Available:
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2012-05-01
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| Most Recent Update:
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2016-07-12
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