| HMS LINCS ID:
|
50033
|
| Name:
|
NCI-H1651
|
| Alternative Names:
|
H1651
|
| LINCS ID:
|
LCL-1633
|
| Alternative ID:
|
CLO_0008008
|
| Parent Cell Line:
|
|
| Reference Source:
|
ATCC CRL-5884
|
| Organism:
|
Homo sapiens
|
| Organ:
|
lung
|
| Tissue:
|
|
| Cell Type:
|
epithelial
|
| Details of Cell Type:
|
|
| Donor Sex:
|
male
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:3910, lung adenocarcinoma
|
| Details of Disease:
|
NSCLC adenocarcinoma
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
COSS910900
|
| Verification Reference Profile:
|
DNA Profile (STR, source: ATCC): Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 11
D5S818: 12,14
D7S820: 8
THO1: 8
TPOX: 11
vWA: 18
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: ACL-4 medium supplemented with 10% FBS. ACL-4 medium is formulated at the ATCC as follows:
A 1:1 mixture of Ham's F12 medium with 2.5 mM L-glutamine and Dulbecco's Modified Eagle's Medium adjusted to contain 1.2 g/L sodium bicarbonate (available as ATCC 30-2006) with additional supplements at the concentrations below:
0.02 mg/ml insulin
0.01 mg/ml transferrin
25 nM sodium selenite
50 nM Hydrocortisone
1 ng/ml Epidermal Growth Factor (do not filter)
0.01 mM ethanolamine
0.01 mM phosphorylethanolamine
100 pM triiodothyronine
0.5% (w/v) bovine serum albumin
10 mM HEPES
0.5 mM sodium pyruvate
2mM L-glutamine
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-04-04
|