| HMS LINCS ID:
|
50030
|
| Name:
|
MDA-MB-435S
|
| Alternative Names:
|
|
| LINCS ID:
|
LCL-1307
|
| Alternative ID:
|
CLO_0007638
|
| Parent Cell Line:
|
|
| Reference Source:
|
ATCC HTB-129
|
| Organism:
|
Homo sapiens
|
| Organ:
|
skin
|
| Tissue:
|
|
| Cell Type:
|
melanocyte
|
| Details of Cell Type:
|
|
| Donor Sex:
|
female
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:1909, malignant melanoma (updated based on http://www.atcc.org/products/all/HTB-129.aspx)
|
| Details of Disease:
|
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
|
| Verification Reference Profile:
|
DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 13
D5S818: 12
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 16,18
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 0.01 mg/ml insulin, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, add fresh 0.25%trypsin - 0.053 mM EDTA, rinse and remove. Place flask at room temperature (or incubated at 37C) for approximately 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-07-12
|