MCF7 - Cell Line (ID:50029)

Name: MCF7
Alternative Names:
Alternative ID: CLO_0007606
Parent Cell Line:
Reference Source: ATCC HTB-22
Organism: Homo sapiens
Organ: breast
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3458, adenocarcinoma
Details of Disease: breast adenocarcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS905946
Verification Reference Profile: DNA Profile (STR, source: ATCC): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11,12 D5S818: 11,12 D7S820: 8,9 THO1: 6 TPOX: 9,12 vWA: 14,15
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 90%; fetal bovine serum, 10%. Protocol: Remove culture medium to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer the cell suspension to the centrifuge tube with the medium and cells from step 1, and centrifuge at approximately 125 x g for 5 to 10 minutes. Discard the supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-04-25
Most Recent Update: 2016-04-04

Batch Information for HMSL50029-2:

HMS LINCS Batch ID: 50029-2
Provider: ATCC
Provider Catalog ID: HTB-22
Provider Batch ID:
Source Information: Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010.
Date Received: 2010-07-16
HMS QC Outcome: PASS
Transient Modification(s):
Date Publicly Available: 2016-06-06
Most Recent Update: 2016-09-09

HMS QC Testing Events for HMSL50029-2:

Date HMS QC Outcome Comment Files
06/30/2015 PASS Chung_STR_Profiling_Results_6_30_15.xls