| HMS LINCS ID:
|
50027
|
| Name:
|
KYSE-450
|
| Alternative Names:
|
|
| LINCS ID:
|
LCL-1550
|
| Alternative ID:
|
CLO_0007134
|
| Parent Cell Line:
|
|
| Reference Source:
|
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 387
|
| Organism:
|
Homo sapiens
|
| Organ:
|
esophagus
|
| Tissue:
|
|
| Cell Type:
|
epithelioid
|
| Details of Cell Type:
|
|
| Donor Sex:
|
male
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:3748, esophageal squamous cell carcinoma
|
| Details of Disease:
|
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
COSS907320
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: 45% RPMI 1640 + 45% Ham's F12 + 10% FBS split confluent culture 1:8 to 1:10 every 3-4 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm 2 at 37 C with 5% CO2 cell harvest of ca. 5 x 106 cells/25 cm 2
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-08-24
|