| HMS LINCS ID:
|
50024
|
| Name:
|
KYSE-140
|
| Alternative Names:
|
|
| LINCS ID:
|
LCL-1547
|
| Alternative ID:
|
CLO_0007128
|
| Parent Cell Line:
|
|
| Reference Source:
|
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 348
|
| Organism:
|
Homo sapiens
|
| Organ:
|
esophagus
|
| Tissue:
|
|
| Cell Type:
|
|
| Details of Cell Type:
|
|
| Donor Sex:
|
male
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:3748, esophageal squamous cell carcinoma
|
| Details of Disease:
|
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
COSS753573
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: 90% RPMI 1640 + 10% FBS split confluent culture 1:5 to 1:10 every 2-4 days using trypsin/EDTA (cells may need five or more minutes to detach from the flask; prior to using trypsin, cells should be washed twice with PBS); seed out at ca. 2-3 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of ca. 20-30 x 10 6 cells/175 cm 2
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-08-24
|