KYSE-140 - Cell Line (ID:50024)
| HMS LINCS ID: | 50024 |
| Name: | KYSE-140 |
| Alternative Names: | |
| LINCS ID: | LCL-1547 |
| Alternative ID: | CLO_0007128 |
| Parent Cell Line: | |
| Reference Source: | Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 348 |
| Organism: | Homo sapiens |
| Organ: | esophagus |
| Tissue: | |
| Cell Type: | |
| Details of Cell Type: | |
| Donor Sex: | male |
| Donor Age: | |
| Donor Ethnicity: | |
| Donor Health Status: | |
| Disease: | DOID:3748, esophageal squamous cell carcinoma |
| Details of Disease: | |
| Production Details: | |
| Genetic Modification(s): | none |
| Known Mutations: | |
| Citation Information for Mutations: | COSS753573 |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | From MGH/CMT as specified by cell provider: 90% RPMI 1640 + 10% FBS split confluent culture 1:5 to 1:10 every 2-4 days using trypsin/EDTA (cells may need five or more minutes to detach from the flask; prior to using trypsin, cells should be washed twice with PBS); seed out at ca. 2-3 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of ca. 20-30 x 10 6 cells/175 cm 2 |
| Relevant Citations: | |
| Usage Note: | |
| Comments: | |
| Date Publicly Available: | 2012-04-25 |
| Most Recent Update: | 2016-08-24 |
Datasets:
| HMS Dataset ID | Dataset Title | HMS Dataset Type |
|---|---|---|
| 20002 | Moerke 3 Color Apoptosis | Microscopy/Imaging |
| 20003 | Tang Mitosis/Apoptosis ver. II | Microscopy/Imaging |
| 20004 | Tang Proliferation/Mitosis | Microscopy/Imaging |
Batch Information:
| HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
|---|---|---|---|
| 50024-1 | Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) | MGH-496 | |
| 50024-2 | Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) | MGH-496 |