| HMS LINCS ID:
|
50018
|
| Name:
|
Ishikawa
|
| Alternative Names:
|
|
| LINCS ID:
|
LCL-1497
|
| Alternative ID:
|
CLO_0037163
|
| Parent Cell Line:
|
|
| Reference Source:
|
European Collection of Authenticated Cell Cultures (ECACC) 99040201
|
| Organism:
|
Homo sapiens
|
| Organ:
|
uterus
|
| Tissue:
|
|
| Cell Type:
|
epithelial-like
|
| Details of Cell Type:
|
|
| Donor Sex:
|
female
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:363, uterine cancer; DOID:1380, Endometrial cancer; DOID:2871, endometrial carcinoma; DOID:2870, endometrial adenocarcinoma
|
| Details of Disease:
|
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
|
| Verification Reference Profile:
|
STR-PCR Data (source: ECACC):
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
ECACC growth medium: MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS). Subculture routine: Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 deg C; cells tend to pile up.
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-08-24
|