CAL-51 - Cell Line (ID:50008)

HMS LINCS ID: 50008
Name: CAL-51
Alternative Names:
LINCS ID: LCL-1472
Alternative ID: CLO_0002185
Parent Cell Line:
Reference Source: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 302
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type: epithelial-like
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3459, breast carcinoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS910927
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: 80% Dulbecco's MEM (4.5 g/L glucose) + 20% FBS split culture 1:2 once or twice a week using trypsin/EDTA before cells reach complete confluency; seed out at ca. 2 x 106 cells/25 cm 2 ; cells may grow very slowly initially after thawing or after stringent trypsination at 37C with 5-10% CO2 cell harvest of ca. 15-20 x 106 cells/80 cm 2
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-04-25
Most Recent Update: 2016-08-24

Batch Information for HMSL50008-2:

HMS LINCS Batch ID: 50008-2
Provider: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures
Provider Catalog ID: ACC-302
Provider Batch ID:
Source Information: Obtained by Caitlin Mills (Harvard Medical School) from DSMZ
Date Received:
HMS QC Outcome:
Transient Modification(s):
Comments:
Date Publicly Available: 2018-02-09
Most Recent Update: 2018-02-09