| HMS LINCS ID:
|
50008
|
| Name:
|
CAL-51
|
| Alternative Names:
|
|
| LINCS ID:
|
LCL-1472
|
| Alternative ID:
|
CLO_0002185
|
| Parent Cell Line:
|
|
| Reference Source:
|
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 302
|
| Organism:
|
Homo sapiens
|
| Organ:
|
breast
|
| Tissue:
|
|
| Cell Type:
|
epithelial-like
|
| Details of Cell Type:
|
|
| Donor Sex:
|
female
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:3459, breast carcinoma
|
| Details of Disease:
|
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
COSS910927
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: 80% Dulbecco's MEM (4.5 g/L glucose) + 20% FBS split culture 1:2 once or twice a week using trypsin/EDTA before cells reach complete confluency; seed out at ca. 2 x 106 cells/25 cm 2 ; cells may grow very slowly initially after thawing or after stringent trypsination at 37C with 5-10% CO2 cell harvest of ca. 15-20 x 106 cells/80 cm 2
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-08-24
|