| HMS LINCS ID:
|
50005
|
| Name:
|
BPH-1
|
| Alternative Names:
|
|
| LINCS ID:
|
LCL-2095
|
| Alternative ID:
|
CLO_0002022
|
| Parent Cell Line:
|
|
| Reference Source:
|
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 143
|
| Organism:
|
Homo sapiens
|
| Organ:
|
prostate
|
| Tissue:
|
|
| Cell Type:
|
epithelioid
|
| Details of Cell Type:
|
|
| Donor Sex:
|
male
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:0060087, male reproductive benign neoplasm
|
| Details of Disease:
|
benign prostate hyperplasia
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
COSS924105
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: 80% RPMI 1640 + 20% FBS + 20 ng/ml testosterone + 5 microg/ml transferrin + 5 ng/ml sodium selenite + 5 microg/ml insulin + trace elements mix (a list can be provided as they are no longer commercially available in a single mix) split confluent culture 1:3 to 1:8 every 3-7 days using trypsin/EDTA (cells should be incubated for more than 10 min with trypsin/EDTA at 37C); seed out at 0.1 x 10 5 cells/cm 2 ; we noted that the cells grow equally well without the trace element mix which was recommended by the depositor at 37C with 5% CO2 cell harvest of ca. 0.4 x 105 cells/cm 2
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-08-24
|