| HMS LINCS ID:
|
50002
|
| Name:
|
647-V
|
| Alternative Names:
|
|
| LINCS ID:
|
LCL-1708
|
| Alternative ID:
|
CLO_0001450
|
| Parent Cell Line:
|
|
| Reference Source:
|
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 414
|
| Organism:
|
Homo sapiens
|
| Organ:
|
urinary bladder
|
| Tissue:
|
|
| Cell Type:
|
epithelial-like
|
| Details of Cell Type:
|
|
| Donor Sex:
|
male
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:4007, bladder carcinoma; DOID:4006, bladder transitional cell carcinoma
|
| Details of Disease:
|
transitional cell carcinoma; urothelial bladder carcinoma
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
COSS906797
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: 85% Dulbecco's MEM + 15% FBS + 2 mM L-glutamine split confluent culture 1:5 to 1:6 every 3-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5-10% CO2 cell harvest of ca. 10-15 x 106 cells/175 cm 2
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-08-24
|