5637 - Cell Line (ID:50001)

Name: 5637
Alternative Names:
Alternative ID: CLO_0001421
Parent Cell Line:
Reference Source: ATCC HTB-9
Organism: Homo sapiens
Organ: urinary bladder
Cell Type: epithelial
Details of Cell Type:
Donor Sex: male
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:4007, bladder carcinoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS687452
Verification Reference Profile: DNA Profile (STR, source: ATCC): Amelogenin: X,Y CSF1PO: 11 D13S317: 11 D16S539: 9 D5S818: 11,12 D7S820: 10,11 THO1: 7,9 TPOX: 8,9 vWA: 16,18
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-04-25
Most Recent Update: 2016-04-04

Batch Information for HMSL50001-2:

HMS LINCS Batch ID: 50001-2
Provider: Center for Molecular Therapeutics (CMT; Massachusetts General Hospital)
Provider Catalog ID: MGH-6
Provider Batch ID:
Source Information: Obtained by Yangzhong Tang (Harvard Medical School) from the Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) (contact: Cyril Benes).
Date Received:
HMS QC Outcome:
Transient Modification(s):
Date Publicly Available: 2016-06-06
Most Recent Update: 2016-07-12