H2A.X (pS139) / clone 20E3 / Alexa Fluor 647 - Antibody (ID:80030)

HMS LINCS ID: 80030
Name: H2A.X (pS139) / clone 20E3 / Alexa Fluor 647
Alternative Names: Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 647 Conjugate) #9720
Clone Name: 20E3
Resource Identification Initiative ID: AB_10692910
LINCS ID:
Type: natural
Nominal Protein Target(s): H2A.X (pS139)
Nominal Protein Target HMS LINCS ID(s): 201314
Nominal Non-protein Target:
Organism of Nominal Target: Homo sapiens
Other Target Information: Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb detects endogenous levels of H2A.X only when phosphorylated at serine 139. Species Reactivity: Human, Mouse, Rat, Monkey. (source: Cell Signaling Technology)
Other Human Protein Target HMS LINCS ID(s):
Immunogen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X. (source: Cell Signaling Technology)
Immunogen Sequence:
Fc Domain Source Species: rabbit
Clonality: monoclonal
Fc Domain Isotype: IgG
Source Organism for Production: rabbit
Production Details:
Conjugate or Label: Alexa Fluor 647
Labeling Details: The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-5. (source: Cell Signaling Technology)
Relevant Citations:
Comments:
Date Publicly Available: 2016-09-14
Most Recent Update: 2016-09-14

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20267
Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with EGF or four kinase inhibitors
Microscopy/Imaging

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
80030-1 Cell Signaling Technology 9720S 1