H3 (pS10) - Antibody (ID:80001)
| HMS LINCS ID: | 80001 |
| Name: | H3 (pS10) |
| Alternative Names: | Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475 |
| Clone Name: | D2C8 |
| Resource Identification Initiative ID: | AB_10694639 |
| LINCS ID: | |
| Type: | natural |
| Nominal Protein Target(s): | H3 (pS10) |
| Nominal Protein Target HMS LINCS ID(s): | 201294 |
| Nominal Non-protein Target: | |
| Organism of Nominal Target: | Homo sapiens |
| Other Target Information: | Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of histone H3 only when phosphorylated at Ser10. The antibody does not cross-react with other phosphorylated histones or with acetylated histones. Species Reactivity: Human, Mouse, Rat, Monkey, Zebrafish. (source: Cell Signaling Technology) |
| Other Human Protein Target HMS LINCS ID(s): | |
| Immunogen: | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3. (source: Cell Signaling Technology) |
| Immunogen Sequence: | |
| Fc Domain Source Species: | rabbit |
| Clonality: | monoclonal |
| Fc Domain Isotype: | IgG |
| Source Organism for Production: | rabbit |
| Production Details: | |
| Conjugate or Label: | Alexa Fluor 555 |
| Labeling Details: | This antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6. (source: Cell Signaling Technology) |
| Relevant Citations: | |
| Comments: | |
| Date Publicly Available: | 2015-09-16 |
| Most Recent Update: | 2016-09-14 |
Datasets:
| HMS Dataset ID | Dataset Title | HMS Dataset Type |
|---|---|---|
| 20236 | Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line | Microscopy/Imaging |
| 20267 | Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with EGF or four kinase inhibitors | Microscopy/Imaging |
| 20303 | LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 1 of 6: Cytosolic fluorescent intensity measurements at 24 hr. | Microscopy/Imaging |
| 20304 | LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 2 of 6: Nuclear fluorescent intensity measurements at 24 hr. | Microscopy/Imaging |
| 20305 | LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 3 of 6: Cytosolic fluorescent intensity measurements at 48 hr. | Microscopy/Imaging |
| 20306 | LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 4 of 6: Nuclear fluorescent intensity measurements at 48 hr. | Microscopy/Imaging |
| 20307 | LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 5 of 6: Cytosolic fluorescent intensity measurements at 72 hr. | Microscopy/Imaging |
| 20308 | LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 6 of 6: Nuclear fluorescent intensity measurements at 72 hr. | Microscopy/Imaging |
Batch Information:
| HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
|---|---|---|---|
| 80001-1 | Cell Signaling Technology | 3475S | 2 |