Live-cell imaging of cell-to-cell variability in caspase-8 FRET reporter activity and cell fate trajectory modeling - Dataset (ID:20232)

Data Columns

  • 19 Data Columns
Name Description Display Name Data Type Unit Precision Readout Type Replicate Comments
2ndAntibodyConcentration
Concentration of antibody clustering agent
2nd Antibody Concentration Numeric 1
2ndAntibodyConcUnit 2nd Antibody Conc Unit Text
2ndAntibodyName 2nd Antibody Name Text
antibodyConcentration
Concentration of antibody agonist of DR4 or DR5
Antibody Concentration Numeric 1
antibodyConcUnit Antibody Conc Unit Text
antibodyName Antibody Name Text
cell
Cell reagent
Cell cell
classificationaccuracyFateBoundary
Fraction of cells properly classified as viable or inviable 10 hours after treatment based on the landscape of parameters k and tau
Classification Accuracy (fate boundary) Numeric 5
classificationaccuracyThetaT
Fraction of cells properly classified as viable or inviable 10 hours after treatment based on FRET reporter activation
Classification Accuracy (theta_T) Numeric 5
meanlog10KAU
Average of the calculated log10(k) values (in arbitrary units) across all individual cells analyzed for a given treatment
Mean log10(k) (a.u.) Numeric 2
meantauMin
Average of the calculated tau values (in min) across all individual cells analyzed for a given treatment
Mean tau (min) Numeric 4
protein
Protein perturbagen
Protein protein
proteinConcentration
Concentration of DR4/5 agonist
Protein Concentration Numeric 1
proteinConcUnit Protein Conc Unit Text
replicate Replicate Numeric 0
smallMolConcentration Small Mol Concentration Numeric 0
smallMolConcUnit Small Mol Conc Unit Text
smallMolecule
Small molecule perturbagen
Small Molecule small_molecule
survivingFraction
Fraction of cells scored as viable 10 hours after treatment based on morphology
Surviving Fraction Numeric 3
  • 19 Data Columns